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| Montealegre,Jaime; Valderrama,Luis; Herrera,Rodrigo; Besoaín,Ximena; Pérez,Luz M. |
| Wild (Th11, Th12 and Th650) and mutant (Th11A80.1, Th12A40.1, Th12C40.1 and Th650-NG7) Trichoderma harzianum strains were stored for 180 days at 5ºC or at 22ºC, in two types of soils. Strains recovered at 90, 120 and 180 days from the two types of soils, retained their full capacity to biocontrol Rhizoctonia solani 618, that produces crown and root rot of tomatoes. Recovery, estimated as colony forming units (cfu) of the wild and mutant strains, showed that all increased their cfu after storage independently of the type of soil and temperature, although kinetic behavior differed among strains. Ratios of recovery after storage in type B soil/ type A soil or at 22ºC/5ºC, higher or lower than one respectively, allowed to establish that Th11 and Th12 were the... |
| Tipo: Journal article |
Palavras-chave: Bioantagonism; Persistence; Root rot; Tomato. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400002 |
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| Montealegre,Jaime; Valderrama,Luis; Sánchez,Soledad; Herrera,Rodrigo; Besoain,Ximena; Pérez,Luz María. |
| Biocontrol of Rhizoctonia solani in tomatoes cultivated under greenhouse and field conditions was analyzed using the Trichoderma harzianum mutants Th650-NG7, Th11A80.1, Th12A40.1, Th12C40.1 and Th12A10.1 and ThF2-1, respectively. Their innocuousness on tomato cultivars 92.95 and Gondola (greenhouse assays), and on cultivar Fortaleza (field assays) was established. Alginate pellets (1.7 g pellets/L soil) containing c.a1 x 10(5) colony forming units (cfu)/g pellet were applied to a soil previously inoculated with R. solani at transplant (greenhouse) or to a naturally infected soil (field). Controls considered parental wild strains, a chemical fungicide and no additions. Th11A 80.1, Th12A10.1 and Th650-NG7 prevented the 100% mortality of tomato plants cv.... |
| Tipo: Journal article |
Palavras-chave: Bioantagonism; Field assays; Greenhouse assays; Root rot; Tomato. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200001 |
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| Polanco,Rubén; Pino,Constanza; Besoain,Ximena; Montealegre,Jaime; Pérez,Luz M. |
| The secretion of enzymes involved in biocontrol from the wild T. harzianum strains Th11 and Th12 and the mutants Th11A80.1, Th11C40.1 and Th12A10.1 was studied after their cultivation in the presence of R. solani cell walls as the sole carbon source. The results showed that endoprotease activity in the supernatants from Th11A80.1 and Th11C40.1 increased 2.77 and 4.79-fold, respectively, and the P-1,3-glucanase and P-1,4-chitinase activity from Th12A10.1 increased 1.86 and 1.54-fold, respectively, compared with the corresponding parental strains. The role of the three enzymes in the biocontrol of R. solani is discussed. |
| Tipo: Journal article |
Palavras-chave: SS-1; 3-glucanases endoproteases ß-1; 4-chitinases. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000200010 |
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| Herrera,Rodrigo; Núñez,David; Romero,Natalia; Besoain,Ximena; Pérez,Luz María; Montealegre,Jaime. |
| The germination of conidia in wild-type (Th11, Th12 and Th650) and mutant (Th11A80.1, Th12A10.1 and Th650-NG7) strains of Trichoderma harzianum that were exposed to different commercial fungicides was studied. All wild-type and mutant Trichoderma strains were germinated in the presence of 1,700 mg L-1 of pencycuron. The wild-type strains Th12 and Th650 and the corresponding mutant strains Th12A10.1 and Th650-NG7 were sensitive to all concentrations of iprodione and metalaxil + mancozeb. The EC50 (Median Effective Concentration) values for the different fungicides were between 10-1 and 10-4 less than the concentrations recommended for field applications; one exception was Phyto-fos on Th650-NG7, where this ratio was 0.72. These results establish whether... |
| Tipo: Journal article |
Palavras-chave: Biocontrol; Biocontrol agents; Control of tomato root diseases; Fungicides; Fungicide tolerant Trichoderma; Integrated management. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300016 |
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